Author Zhichao Xu, Yuan Liu, Yongchang Cao
Porcine epidemic diarrhea virus (PEDV) causes severe diarrhoea and high mortality in neonatal suckling piglets, leading to significant economic losses to the swine industry, urgent need to exploit effect antiviral therapies to compensate for vaccines, Aloe extract can hamper completely the proliferation of PEDV at a non-cytotoxic concentration of 16 mg/mL determined by CCK-8 assay in Vero and IPEC-J2 cells in vitro, Aloe extract efficiently inhibited PEDV infection in vivo, aqueous extract from the Aloe could inhibit PEDV replication in vitro and in vivo and might be a good target for drug development against PEDV, Aloe extract (Ae) had the ability to inhibit PEDV infection, first examined its potential to inhibit PEDV replication in vitro and determined the stages in the PEDV life cycle that could be blocked by Aloe vera extract , and then extended our study in newborn piglets at a relatively safety concentration to see if Aloe vera extract could inhibit PEDV infection in vivo, Aloe vera can be potentially useful for the development of anti-PEDV therapeutics, Immunofluorescence assay, Determination of direct virion inactivation activity of Aloe vera gel
