Author: Thu Han Le , Phan Se Yong, Park Hyun , Jin Jung, Seung Hyun Oh
tight junction-modulating effects of processed Aloe vera gel (PAG), comprising 5400-kD polysaccharides as the main components, oral treatment of 143 mg/kg Polysaccharides Aloe Gel daily for 10 days improves the age-related leaky gut condition in old mice, by reducing their individual urinal lactulose/mannitol (L/M) ratio. In concordance with in vivo experiments, Polysaccharides Aloe Gel treatment at dose 400 ?g/mL accelerated the polarization process of Caco-2 monolayers. The underlying mechanism was attributed to enhancement in the expression of intestinal tight junction-associated scaffold protein zonula occludens (ZO)-1 at the translation level. This was induced by activation of the MAPK/ERK signaling pathway, which inhibits the translation repressor 4E-BP1. In conclusion, we propose that consuming PAG as a complementary food has the potential to benefit high-risk patients, Intestinal tight junctions are complex structures containing various intercellular membrane proteins (such as claudins and occludin) attached to the scaffolding zonula occludens proteins (ZOs), and through them, to the cytoskeleton actin and myosin of intestinal epithelial cells. Together, they form a barrier that dictates the paracellular absorption of electrolytes and macro-molecules, thereby contributing to safeguarding our body against a bacteria-laden intestinal lumen, leaky gut is assumed to originate due to compromised intestinal tight junction integrity, polysaccharides of Aloe vera gel are likely to possess bioactivity to help alleviate the leaky gut condition, Polysaccharides Aloe Gel increases Tight Junction Proteins of Intestinal Epithelial Cells
